Composite
Part:BBa_K2036010:Design
Designed by: Zhangyu Cheng Group: iGEM16_HUST-China (2016-09-19)
Cro-TT-pRM-RBS-GFP-LVAssrAtag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1036
Design Notes
One should make sure there is an RBS when constructing the expression plasmid else the repression effect will not be significant.
Source
GFP-LVA tag and TT are all standards parts from Registry. And Cro pRM are from bacteriaphage lambda.