Composite

Part:BBa_K2036010:Design

Designed by: Zhangyu Cheng   Group: iGEM16_HUST-China   (2016-09-19)


Cro-TT-pRM-RBS-GFP-LVAssrAtag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1036


Design Notes

One should make sure there is an RBS when constructing the expression plasmid else the repression effect will not be significant.


Source

GFP-LVA tag and TT are all standards parts from Registry. And Cro pRM are from bacteriaphage lambda.

References